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KMID : 0903519680100010023
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1968 Volume.10 No. 1 p.23 ~ p.31
Studies on Cellulolytic Enzymes Produced by Chaetomium globosum


Abstract
We have obtained the following results, at the production of cellulase of Chaetomium globosum and its properties of crude enzyme.
1. At the production of enzyme, wheat bran solid culture was more active than surface or shaking culture.
2. The production of enzyme was maximum between the eighth and the tenth days, but slightly decreased thereafter.
3. The optimum condition of the reactions in saccharification with CMC were obtained the following results.
1) The optimum pH was within the range of from 4.0 to 5.0 and stable pH range was within 3.5 to 6.5.
2) The optimum temperature was 40¡É and thermal stability was below 50¡É and completely inactivated at 70¡É
4 Dialyzed crude enzyme was activated by Mn^(++) Mg^(++) Fe^(++) and Mo^(++) respectively but Hg^(++) was inhibited its enzyme action.
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